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rnp complex  (New England Biolabs)


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    Structured Review

    New England Biolabs rnp complex
    Rnp Complex, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnp+complex/EnGen+Spy+Cas9+NLS/pm41903735-47-1-14
    Average 99 stars, based on 599 article reviews
    rnp complex - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Insights into the compact CRISPR-Cas9d system.
    Article Snippet: .. The purified RNP complex was pre-incubated with supercoiled or FspI (New England Biolabs, R0135L) digested linearized target plasmidDNA for 15min at 37 °C. ..

    Article Title: Insights into the compact CRISPR–Cas9d system
    Article Snippet: .. The purified RNP complex was pre-incubated with supercoiled or FspI (New England Biolabs, R0135L) digested linearized target plasmid DNA for 15 min at 37 °C. ..

    In Vitro:

    Article Title: Nonlinear control of transcription through enhancer-promoter interactions
    Article Snippet: .. In vitro digestion and A-tailing of the DNA were performed by adding 10uL of the RNP complex, 10mM of dATP (NEB, N0440) and 5U of Taq Polymerase (NEB, M0267) and incubating the samples at 30min 37°C followed by 5min 72°C. ..

    Article Title: Live-cell imaging and physical modeling reveal control of chromosome folding dynamics by cohesin and CTCF
    Article Snippet: .. In vitro digestion and A-tailing of the DNA were performed by adding 10 μl of the RNP complex, 10 mM of dATP (NEB, N0440) and 5 U of Taq Polymerase (NEB, M0267) and incubating the samples at 30 min, 37°C followed by 5 min, 72°C. .. Nanopore sequencing adaptors were ligated using the Ligation Sequencing Kit (Oxford Nanopore Technologies, SQK-CAS109) according to the manufacturer’s instructions.

    Article Title: Nonlinear control of transcription through enhancer-promoter interactions.
    Article Snippet: .. In vitro digestion and A-tailing of the DNA were performed by adding 10 μl of the RNP complex, 10 mM of dATP (NEB, N0440) and 5 U of Taq Polymerase (NEB, M0267) and incubating the samples for 30 min at 37 °C followed by 5 min at 72 °C. ..

    Article Title: Nonlinear control of transcription through enhancer–promoter interactions
    Article Snippet: .. In vitro digestion and A-tailing of the DNA were performed by adding 10 μl of the RNP complex, 10 mM of dATP (NEB, N0440) and 5 U of Taq Polymerase (NEB, M0267) and incubating the samples for 30 min at 37 °C followed by 5 min at 72 °C. ..

    Plasmid Preparation:

    Article Title: Insights into the compact CRISPR–Cas9d system
    Article Snippet: .. The purified RNP complex was pre-incubated with supercoiled or FspI (New England Biolabs, R0135L) digested linearized target plasmid DNA for 15 min at 37 °C. ..



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